Extended Data Fig. 2: (A) Mouse IELp do not express GPR15 or CCR9 at the protein level.
From: An SSTR2–somatostatin chemotactic axis drives T cell progenitor homing to the intestines
Flow cytometric analysis of P1 neonatal mouse thymocytes confirms no classic gut homing GPCR expression by gut homing (α4β7+) mouse IELp. Neonatal TCRγδ cell subsets are used as positive internal controls for GPR15 and CCR9 staining. (B) Single and double positive mouse thymocytes do not migrate to SST or CORT. In vitro migration results of thymocyte subsets to CXCL12 (100 nM) as positive control, as well as SST-28 (1uM) and CORT-14 (100 nM). Data are shown as mean ± SEM (n = 6 to 8 per group) and are representative of 2 independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
