Fig. 6: Tuft cell-intrinsic IL-17RB regulates tonic IL-25 bioavailability. | Nature Immunology

Fig. 6: Tuft cell-intrinsic IL-17RB regulates tonic IL-25 bioavailability.

From: Tuft cell IL-17RB restrains IL-25 bioavailability and reveals context-dependent ILC2 hypoproliferation

Fig. 6: Tuft cell-intrinsic IL-17RB regulates tonic IL-25 bioavailability.The alternative text for this image may have been generated using AI.

a, Flow cytometry analysis of Ki-67 and Sm13 expression by small intestinal ILC2s from 3-week-old mice of the indicated genotypes. b, Quantification of IL-13 (Sm13)+ ILC2s (n = 6–9 mice). c, Analysis of KLRG1 expression by ILC2s. d, Quantification of KLRG1 MFI in ILC2s (n = 6–9 mice). e, Expression of tdTomato and CD24 in CD45low/–EpCAM+ cells determined by flow cytometry analysis from 3-week-old Vil1Cre; Il25fl/fl and Il25fl/fl mice, which also encode the Il25tdTomato reporter, and compared to that in a reporter-negative mouse. f,g, Frequencies of IL-13 (Sm13)+ (f) and Ki-67+ (g) ILC2s (n = 10–11 mice). h, Quantification of the KLRG1 MFI in ILC2s (n = 10–11 mice). i, Quantification of tuft cells by flow cytometry (n = 10–11 mice). j, Experimental scheme. k, Flow cytometry analysis of Ki-67 and Sm13 reporter expression by ILC2s from young Vil1Cre; Il17rbfl/fl and Il17rbfl/fl mice after five injections of PBS or an anti-IL-17RB blocking antibody. l, Quantification of IL-13 (Sm13)+ ILC2s (n = 9–11 mice). Data are indicated as mean or mean ± s.e.m (b, d, f, g, i, l). Data were analyzed using an ordinary one-way ANOVA (b, d, l) or the Mann–Whitney test (f, g, h, i). *P = 0.01–0.05; **P = 0.01–0.001; ****P < 0.0001; NS, P ≥ 0.05.

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