Fig. 4: Impairment of monocyte maturation in the late immunosuppressive phase after LPS administration.
From: Systemic inflammation impairs myelopoiesis and interferon type I responses in humans
a, UMAP representation of HSCs and myeloid lineage cells from bone marrow and peripheral blood of LPS-challenged healthy volunteers (n = 3 for each compartment) at BL (bone marrow, 7 d before the LPS challenge; blood, immediately before the LPS challenge) and at 4 h and d7 post-LPS challenge, colored based on cell type. b, UMAP of all blood and bone marrow cells (n = 3) as in a, colored based on compartment. c, UMAP of all blood and bone marrow cells (n = 3) as in a, colored based on time point. d, Nearest neighbor graph-based, differential abundance analysis between BL and d7 of HSCs and myeloid cells from bone marrow and blood of LPS-challenged healthy volunteers (n = 3 for each compartment). The blue circles represent significantly reduced neighborhoods on d7. e, Heatmap representation of NMF-inferred genes highly expressed in iMonos and ncMonos in cells as in d (n = 3 for each compartment). f, UMAP representation of enrichment of ncMono and IFN-I gene programs in cells as in d. g, Dot plot representation of log2(abundance change) versus log2(average expression change) of ncMono and IFN-I gene programs on d7 versus BL in cells as in d.
