Extended Data Fig. 3: Binding of NF-κB dimers to Il12b motifs.
From: Stepwise neofunctionalization of the NF-κB family member Rel during vertebrate evolution
a, EMSAs were performed to examine the relative binding strength of various NF-κB RHR homodimers and heterodimers to a radiolabeled probe containing the Il12b κB3 sequence. For heterodimers, two NF-κB family members were co-expressed in E. coli. Coomassie stains of the purified are shown at the bottom. Binding of Rel and RelA homodimers to a probe containing a consensus NF-κB motif is shown at the right as a control. Representative images from two or three independent experiments are shown b, EMSAs were used to examine Rel RHR binding to probes containing substitution mutations in the Il12b κB1, 2, and 3 motifs in a radiolabeled probe containing these three motifs in tandem, as in the WT Il12b promoter. The images are representative of two independent experiments.
