Fig. 3: R. aegyptiacus SI organoids recapitulate the cellular diversity of the native bat intestinal epithelium.
From: Bat organoids reveal antiviral responses at epithelial surfaces
a, Representative bright-field image of R. aegyptiacus-derived SIORG (n = 3). b, UMAP of the integrated R. aegyptiacus-derived SIT, SIORG and differentiated SIORG-DIFF showing the individual cell types. c, Stacked bar plot showing the relative cell-type proportions of cell types in R. aegyptiacus-derived SIT, SIORG and differentiated SIORG-DIFF. d, Immunofluoresence of DAPI nucleus counterstaining (left) and AVIL antibody staining (right) in SIORG from R. aegyptiacus. The arrow points to an individual AVIL+ tuft cell. e, Seurat DotPlot showing the scaled average expression of markers enriched in tuft and brush cells for tuft/brush, and non-tuft/brush, ECs in the R. aegyptiacus-derived nasalALI, bronchialALI, trachealT, lungT, SIORG and SIT scRNA-seq dataset. The dot size represents the percentage of an individual cell type expressing a given marker. Color intensity represents the average expression value. Dot size was set to a maximum percentage of 50%. (Genes expressed by more than 50% of cells have the same dot size.) f, UMAP of EEC subtypes from the integrated R. aegyptiacus SIT + SIORG (left), SIORG (middle) or SIT (right). Cells with an EEC sublineage are color-coded. g, Seurat FeaturePlot showing the average expression of EEC (CHGA) and EEC sublineage marker genes in individual cells of SIORG (top) or SIT (bottom). ARX, differentiated G/I/L/M/X/D EECs; TPH1, enterochromaffin cells; MLN, M cells; PAX4, EEC progenitor cells; SCT, SCT+ S-like cells. The maximum color cutoff for the average expression was set to 3. Cells with an average expression of 3 or greater for a given marker have the same color. Scale bars, 50 µm.
