Extended Data Fig. 3: Irf4–/– BM cell reconstitution does not enhance the generation of other immune cells.
From: IRF4 expression by NK precursors predetermines exhaustion of NK cells during tumor metastasis
(a) Flow cytometry analysis confirming that WT and Irf4–/– BM cells were mixed at a 1:1 ratio prior to transfer into lethally irradiated Rag1–/– mice. (b) Flow cytometry analysis of the percentage of NKP cells in the bone marrow of WT and Irf4–/– mice. n = 3 mice per group; ns, not significant. (c and d) Lethally irradiated Rag1−/− mice were reconstituted with a 1:1 mixture of CD45.1+ WT and CD45.2+ Irf4–/– BM cells. Flow cytometry was performed 6 weeks post transfer to assess the percentage of live cells (c) and CD3+NK1.1– T cells (d) derived from transferred WT or Irf4–/– BM cells. n = 3 mice; for c: ***P = 0.0003, ns, not significant; for d: **P = 0.0052, ns, not significant. Results are representative of two or three independent experiments. Data are presented as mean ± SD (b,c,d). Data were analyzed by a two-tailed unpaired Student’s t-test.
