Fig. 1: CTSL deficiency alters the cTEC pMHCII ligandome and impairs CD4+ T cell positive selection. | Nature Immunology

Fig. 1: CTSL deficiency alters the cTEC pMHCII ligandome and impairs CD4+ T cell positive selection.

From: Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4+ T cells

Fig. 1: CTSL deficiency alters the cTEC pMHCII ligandome and impairs CD4+ T cell positive selection.The alternative text for this image may have been generated using AI.

a, Representative flow cytometry plots of thymocyte subsets in Ctsl+/+ (n = 8) and CtslΔTEC mice (n = 10); frequency ± s.e.m. of CD4SP thymocytes is indicated. b, Percentages ± s.e.m. of thymocyte subsets as in a; NS, not significant; DN, double negative. c, Representative flow plots of lymph node cells from Ctsl+/+ (n = 3) and CtslΔTEC (n = 3) mice; frequency ± s.e.m. of CD4+ T cells is indicated. d, Representative flow cytometry plots of MHCII expression on CD45−EpCAM+Ly51+ cTECs or CD45−EpCAM+Ly51−CD80+ mature mTECs from Ctsl+/+ and CtslΔTEC mice (n = 20 each) and MHCII−/− mice (n = 3 and 2) as background. e, Representative flow plots and mean fluorescence intensity (MFI) ± s.e.m. relative to Ctsl+/+ cells of MHCII:CLIP on cTECs or mature mTECs from Ctsl+/+ (n = 5) and CtslΔTEC mice (n = 5) and MHCII−/− mice (n = 2) as background. f, Representative flow plots and MFI ± s.e.m. relative to Ctsl+/+ cells of MHCII:non-CLIP on cTECs or mature mTECs from Ctsl+/+ (n = 12) and CtslΔTEC mice (n = 13) and MHCII−/− mice as background. g, Representative flow cytometry plots and MFI ± s.e.m. relative to Ctsl+/+ cells of I-Ab:Eα52–68 on cTECs or mature mTECs from Ctsl+/+ (n = 4) and CtslΔTEC (n = 3) mice on the C57BL/6 × BALB/c F1 background and C57BL/6 (Eα−) mice as background controls. h, CD4SP thymocyte percentages ± s.e.m. in Ctsl+/+ mice (n = 6 or 4) or CtslΔTEC mice (n = 5 or 4) reconstituted with BM from MHCII+/+ or MHCII−/− mice. i, CD4SP thymocyte percentages ± s.e.m. in Ctsl+/+ mice (n = 8 or 9) or CtslΔTEC mice (n = 4 or 3) on a wild-type or Ciitakd transgenic background. j, Representative flow cytometry plots of TCRβ and CD69 surface expression on DP cells from B2m−/−Ctsl+/+ (n = 9) and B2m−/−CtslΔTEC mice (n = 13); frequency ± s.e.m. of TCRβintCD69+ cells is shown (P < 0.001). k, Representative flow cytometry analysis and MFI ± s.e.m. relative to Ctsl+/+ cells of CD5 expression on CD4SP thymocytes from Ctsl+/+ and CtslΔTEC mice (n = 7 each). P values in b were determined by two-way analysis of variance (ANOVA) and Sidak’s test for multiple comparisons and in e–k by Student’s two-tailed t-test.

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