Extended Data Fig. 6: Selection in the absence of Ctsl causes aberrant functional tuning and impaired homeostatic fitness of CD4+ T cells. | Nature Immunology

Extended Data Fig. 6: Selection in the absence of Ctsl causes aberrant functional tuning and impaired homeostatic fitness of CD4+ T cells.

From: Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4+ T cells

Extended Data Fig. 6: Selection in the absence of Ctsl causes aberrant functional tuning and impaired homeostatic fitness of CD4+ T cells.The alternative text for this image may have been generated using AI.

a, Cell size (Mean ± SEM) of M2 thymocytes in Ctsl+/+Rag1−/−Lm54Tg (n = 3) and CtslΔTECRag1−/−Lm54Tg mice (n = 4). b, CD44 surface expression (MFI ± SEM) at consecutive DP and CD4SP differentiation stages in Ctsl+/+Rag1−/−Lm54Tg (n = 4) and CtslΔTECRag1−/−Lm54Tg mice (n = 5); assessed by flow cytometry. c, CD69 and CD25 expression (MFI ± SEM) in polyclonal M2 thymocytes from CtslΔTEC (n = 3) or Ctsl+/+ mice (n = 3) after 40 h culture with or without (unstim.) plate-bound antibodies to CD3 and CD28; assessed by flow cytometry. d, Representative flow cytometry plots of intracellular phospho-ERK staining of M2 thymocytes from Ctsl+/+Rag1−/−Lm54Tg (n = 3) and CtslΔTECRag1−/−Lm54Tg mice (n = 3) after 5 min stimulation with PMA. Controls without stimulation define background. e, Donor cell ratio (Mean) in the spleen of 4.5 Gy-irradiated recipients (n = 2) after transfer of a 1:1 mixture of CTV-labelled Ctsl+/+ and CtslΔTEC M2 CD4SPs; assessed by flow cytometry. f, Replication index of donor cells on day 21 after transfer as in (e) assessed with FlowJo_v10.9.0 software, assessed by flow cytometry on the basis of CTV-dilution profiles. P-values by Student’s two-tailed t-test.

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