Extended Data Fig. 2: Ctsl is essential for positive selection of multiple MHCII-restricted transgenic TCRs.
a, Representative flow cytometry plots of thymocyte subsets in Ctsl+/+ and CtslΔTEC mice reconstituted with bone marrow (BM) from the indicated Rag1−/− TCR transgenic donors and corresponding percentages ± SEM of CD4SPs. (n = 3 Ctsl+/+ and 3 CtslΔTEC for LLO56; n = 3 and 3 for LLO118). b, Representative flow cytometry plots and MFI ± SEM of CD5 expression in DP thymocytes from BM chimeras as in (a), relative to cells selected in Ctsl+/+ chimeras. c, Representative flow cytometry plots of thymocyte subsets in Ctsl+/+ Plp1−/− (n = 3) and CtslΔTEC Plp1−/− mice (n = 3) reconstituted with BM from the PLP1 TCR transgenic donors and corresponding percentages ± SEM of CD4SPs. d, Representative flow cytometry plots and MFI ± SEM of CD5 expression in DP thymocytes from BM chimeras as in (c), relative to cells selected in Ctsl+/+ Plp1−/− chimeras. e, CD4SP numbers ± SEM in Ctsl+/+ and CtslΔTEC mice (or Ctsl+/+ Plp1−/− and CtslΔTEC Plp1−/− mice in case of PLP1) reconstituted with BM from the indicated Rag1−/− TCR transgenic donors as in main Fig. 2a or a or c, respectively). All P-values by Student’s two-tailed t-test.
