Extended Data Fig. 5: Proteomic and metabolic profiling of Keap1-/- and WT CD8+ T cells. | Nature Immunology

Extended Data Fig. 5: Proteomic and metabolic profiling of Keap1-/- and WT CD8+ T cells.

From: The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion

Extended Data Fig. 5

(a) Raw peptide intensities for NRF2, GCLM, and ACTN4 in Keap1-/- CD8+ P14 T cells that received Nfe2l2-targeting (sgNfe2l2) or scrambled control (sgScr) sgRNAs, and WT CD8+ P14 T cells that received sgScr sgRNA. (mean±SEM, n = 3). (b-c) Mass isotopologue distribution (MID) for intracellular metabolites from sgNfe2l2 and sgScr Keap1-/-, and sgScr WT P14 T cells following culture with [U-13C]-Glutamine (mean±SEM, n = 4). Shown are the percent labelling (top) and intensities (bottom) for indicated isotopologues for (b) Glutamine, Malate and Citrate, and (c) Glutathione (reduced), γ-glutamyl-cysteine, and Glutamate. (d) Ratio of GGSG:GSH across conditions, taken from total pool values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Back to article page