Extended Data Fig. 6: Immunophenotyping of Keap1-/- sgScr and sgNfe2l2, and WT sgScr P14 cells following LCMV CL13 infection.
From: The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion
(a-e) Bar graphs quantifying (a) the number of P14 (CD45.2+) donor cells and (b) percentage of PD-1hi donor P14 cells. (c) Representative histograms and bar graph of gMFI for PD-1 in CD45.2+ donor cells from (a) at 7 dpi (mean±SEM, n = 3-4/sample). (d) Bar graph quantifying the number of IFN-γhi (CD45.2+/PD-1+) donor cells, corresponding to Fig. 3h. (e) Representative flow cytometry plot of IFN-γ+/TNF-α+ sgNfe2l2 and sgScr Keap1-/-, and sgScr WT CD45.2+/PD-1+ P14 T cells at 7 dpi with quantification of the percentage and number of polyfunctional (IFN-γ+/TNF-α+) PD-1hi sgNfe2l2 and sgScr Keap1-/-, and WT sgScr PD-1+ P14 donor CD8 T cells from the spleen of LCMV CL13-infected mice (7 dpi). (f) Representative flow cytometry plots and representative histograms and bar graph of gMFI for TNF-α, (g) TCF1, and (h) TBET expression by PD-1+ donor cells from (b) at 7 dpi (mean±SEM, n = 3-4/sample). Plots d-f correspond to cells re-stimulated with gp33 (1 μg/mL) peptide in vitro *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001.
