Extended Data Fig. 2: Baseline immunophenotyping of splenocyte populations from Keap1fl/fl (WT) and Cd4Cre+ Keap1fl/fl (Keap1-/-) mice.
From: The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion
(a-b) Percent (top) and number (bottom) of splenic (A) CD3+ T cells and (b) CD8+ (gated on CD3+) T cells from WT and Keap1-/- mice aged 8-12 weeks (mean±SEM, n = 6). (c) Percent (top) and number (bottom) of naïve, effector, central memory, and CD44-/CD62L- (double negative) subsets of CD8+ T cells in the spleens of mice as in (a). (d-e) CD4+ T cells populations in WT and Keap1-/- mice. (d) Percent (top) and number (bottom) of CD4+ (gated on CD3+) T cells from WT and Keap1-/- mice aged 8-12 weeks (mean±SEM, n = 6). (e) Percent (top) and number (bottom) of naïve, effector, central memory, and CD44-/CD62L- (double negative) subsets of CD4+ T cells in the spleens of mice as in (d). (f-h) Percent (top) and number (bottom) of (f) B cells, Natural Killer (NK), and Natural Killer (NKT) T cells, (g) neutrophils, and (h) total CD11c+ dendritic cells (DCs) and conventional DC1 (cDC1) and DC2 (cDC2) subsets from WT and Keap1-/- mice aged 8-12 weeks (mean±SEM, n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
