Extended Data Fig. 10: Analysis of microglia neural transcripts and Y-maze performance.
From: Granzyme K+ CD8 T cells slow tauopathy progression by targeting microglia
a, Representative images from the spinal cord of a P301S−/− CD8WT mouse (n = 3) show Iba1+ myeloid cells (green), NeuN neurons (blue; white asterisks), nuclei (white), and four neural transcripts - Tuba1a (yellow), Map1b (teal), Pcsk1n (pink), and Nefl (orange). The white dotted line outlines an individual Iba1+ myeloid cell. b-c, Two representative images from the spinal cord white matter of P301S+/+ CD8KO mice (n = 5) show Iba1, NeuN, nuclei, Tuba1a, Map1b, Pcsk1n, and Nefl. Multinucleated Iba1+ myeloid cells are outlined with white dotted lines. White arrows denote nuclei within a multinucleated Iba1+ cell that are densely positive for neural transcripts. d, Bar graph shows the slope of the linear regression line for Y-maze performance (triplicate ratios) over a 3-week period for the mouse groups described in Fig. 8f. ns ≥ 0.05, using one-way ANOVA with a Newman Keuls test. e, Bar graph shows the slope of the linear regression line for Y-maze performance (arm entries) over a 3-week period in P301S+/+ mice treated with isotype (n = 7) or anti-PD1/TIGIT (n = 12) antibodies beginning at 21 weeks. P value was calculated using two-tailed Student’s t test. Data are representative of two independent experiments. All dots in d,e represent individual animals, and graphs show mean ± s.d.
