Extended Data Fig. 5: Additional data related to Fig. 5.
From: A STUB1–CHIC2 complex inhibits CD8+ T cells to restrain tumor immunity
(a) Representative histograms (left) and quantification (right) of IL6ST surface expression in activated Control-g1-, Stub1-g1-, or Chic2-g1-nucleofected CD8+ T cells (n = 3, performed twice, one-sided one-way ANOVA with Tukey’s multiple comparisons test). (b-c) Control-g1-, Stub1-g1-, or Chic2-g1-nucleofected naive OT-1 CD8+ T cells were adoptively transferred into mice that were subsequently challenged with B16-OVA tumor cells. Quantification of the MFI of FOXP3 (b) and PD-L1 (c) for tumor-infiltrating OT-1 T cells expressing these markers (n = 10 mice per group, performed twice, one-sided one-way ANOVA with Tukey’s multiple comparisons test). (d) Western Blots of IFNγR1, IL-27Rα, and β-ACTIN (loading control) from Control-g2-nucleofected, Stub1 KO, or Chic2 KO EL4 cells (performed twice). Data represent: mean and s.d. (a-c).
