Extended Data Fig. 1: Additional data related to Fig. 1.
From: A STUB1–CHIC2 complex inhibits CD8+ T cells to restrain tumor immunity
(a) Gating for the naive screen. (b-c) Tumor growth curves for the (b) naive screen (n = 6 groups of 8-10 mice) or (c) activated screen (n = 4 groups of 10 mice). (d-e) Number of recovered transferred T cells in the (d) naive screen or (e) activated screen (Naive screen: n = 6 groups of 8-10 mice and n = 3 input samples, Activated screen: n = 4 groups of 10 mice and n = 2 input samples). (f-g) Number of recovered gRNAs in the (f) naive screen or (g) activated screen. The dotted orange line indicates the maximum gRNAs recoverable (Naive screen: n = 6 groups of 8-10 mice and n = 3 input samples, Activated screen: n = 4 groups of 10 mice and n = 2 input samples). (h-i) Comparison of the log2-normalized fold change in gRNA abundance between the tdLN and input (left) or the tumor and input (right) between replicates in the naive (h) or activated (i) screens. Intraclass correlation coefficient (ICC2) values are listed (n = 6 for the naive screen and n = 4 for the activated screen). (j-l) Screen results for tumor normalized to tdLN. Volcano plots of the median CLES at the gRNA level and q-value at the gRNA-UMI level in the naive screen (j) or activated screen (k). Comparison of the median CLES for the naive and activated screens (l) (Naive screen: n = 6 groups of 8-10 mice, performed twice; Activated screen: n = 4 groups of 10 mice, performed once; Both screens: one-sided FAUST test on gRNA-UMI counts with liberal setting and Benjamini-Hochberg correction). Spearman’s correlation was used to test the significance of correlation. Data represent: mean and s.e.m. (b,c) or mean and s.d. (d-g).
