Extended Data Fig. 3: Loss of p52-ETS1 interaction affects inguinal lymph nodes GC B cells and splenic TFH cell formation but not chronic GCs.
From: The transcription complex p52–ETS1 is essential for germinal center formation
(a) Representative flow cytometry plots and summary bar graphs of frequencies of (b) follicular B cells (CD23+CD21+ CD19+) and (c) marginal zone B cells (CD23− CD21+CD19+) in p52+/+ (Ctrl) and p52ki/ki mice at steady state, n = 10 per group. Pooled data from 3 independent experiments with at least n = 3 mice per group. Summary bar graphs of (d) frequencies and (e) numbers of GC B cell (CD19+ IgD− Fas+ GL7+) assessed by flow cytometry 5 days post intraperitoneal immunization of Ctrl and p52ki/ki mice with NP-OVA and alum, n = 11 per group. Pooled data from 3 independent experiments with at least n = 3 mice per group. f-i, Characterization of chronic GCs in mesenteric lymph nodes (MLN) and Peyer’s patches (PP) of naive Ctrl and p52ki/ki mice by flow cytometry. Summary bar graphs of (f,h) GC B cell frequencies and (g, i) numbers in the MLN and PP respectively. Pooled data from 3 independent experiments with at least n = 3 mice per group. j-k, Ctrl and p52ki/ki mice were immunized subcutaneously with NP-OVA+alum and GCs in inguinal lymph node (ILN) and examined on day 9 by flow cytometry. Summary bar graphs of GC B cell frequencies (j) and numbers (k) in ILN from Ctrl (n = 10) and p52ki/ki (n = 9) mice. Pooled data from 3 independent experiments with minimally n = 3 mice per group. (l) Representative flow cytometry plots of (m) frequencies of splenic TFH cells (CXCR5+PD-1+) 9 days post intraperitoneal immunization of Ctrl (n = 11) and p52ki/ki (n = 10) mice with NP-OVA and alum. Pooled data from 3 independent experiments with minimally n = 3 mice per group. b-k,m, Data are presented as mean ± sd and analysed by two-tailed Mann-Whitney U-tests. Mice were sex matched per experiment. Both male and female mice used in these experiments were 8-12 weeks old.
