Extended Data Fig. 7: Modulation of pDC heterogeneity in bone marrow-derived pDCs by IFN-I blockade.
From: Tonic type I interferon signaling optimizes the antiviral function of plasmacytoid dendritic cells
(a) Total BM cells were harvested from naïve wild-type mice and cultured in the presence of Flt3L for 12 days to induce DC differentiation. Anti-IFNAR1 mAb was added throughout the culture (Blockade), during the first 6 days (D6 washoff) or during the last 6 days (D6 blockade). (b) The dynamics of Sca1 expression on pDCs between days 6 and 12 of culture, as determined by flow cytometry. Cultures from Ifnar1−/− mice were used as controls (dashed line). Data represent averages of biological replicates (cultures from individual mice) from one experiment. (c) The fraction of Sca1+ pDCs on days 6 and 9 of culture. Bars represent means ± SD, symbols represent biological replicates (cultures from individual mice) from one experiment. Statistical significance was analyzed using one-way ANOVA followed by Tukey’s test. * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.00005. Illustration in a created using BioRender.com.
