Fig. 1: Major cell types in the human renal cortex in health and inflammation. | Nature Immunology

Fig. 1: Major cell types in the human renal cortex in health and inflammation.

From: Spatiotemporal interaction of immune and renal cells controls glomerular crescent formation in autoimmune kidney disease

Fig. 1: Major cell types in the human renal cortex in health and inflammation.The alternative text for this image may have been generated using AI.

a, Overview of the patient cohort from the Hamburg Glomerulonephritis Registry and the analysis performed. b, Schematic showing the cross-section of a human kidney tissue with the localization of various cell types in different regions. Inset: cross-section of a glomerulus (top) and tubulointerstitial region (bottom) and the cell types therein. c, DAPI-stained images from kidney biopsies of control (left) and a patient with ANCA-GN (right). The images show glomeruli and surrounding regions overlaid with cell boundaries determined using a cell segmentation algorithm. Localization of specific marker genes is indicated, with glomerular boundaries highlighted for clarity. d, Uniform Manifold Approximation and Projection (UMAP) of ~3.2 million cells retained after QC filtering. Cells are colored based on their annotated cell type. e, Stacked bar plot showing the proportions of different cell types from the complete biopsy tissues across the four disease conditions. f, images from control (left four images) and ANCA-GN (right four images) kidney biopsies showing a glomerulus and tubulointerstitial region. For each region, the adjoining plots show the segmented cells color coded according to their cell-type annotation, illustrating cellular composition differences between conditions in different regions of the tissue. aGBM, anti-GBM antibodies; ATL, ascending thin limb of loop of Henle; bx, biopsies; cDC, conventional dendritic cell; CNT, connecting tubule; cycNKC/T, cycling NK cytotoxic T cell; DCT, distal convoluted tubule; DTL, descending thin limb of loop of Henle; FIB, fibroblast; Fib. MC, fibrotic mesangial cell; IC, intercalated cell of collecting duct; KEGG, Kyoto Encyclopedia of Genes and Genomes; MAC, macrophage; MC, mesangial cell; MDC, monocyte-derived cell; Mono, monocyte; N, neutrophil; NEU, neuronal cell; PpE, papillary tip epithelial cells abutting the calyx; pDC, plasmacytoid dendritic cell; PL, plasma cell; POD, podocyte; PT, proximal tubule; TAL, thick ascending limb of loop of Henle; VSM/P, vascular smooth muscle cells or pericytes.

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