Fig. 4: Foxp3 degradation-sensitive genes in mature T_reg cells are enriched for Foxp3 binding.

a, Bar graphs showing the proportion of ATAC-seq peaks near Foxp3 degradation-induced DEGs bound by Foxp3 (ref. ¹⁹). Genes are stratified by statistical significance (P values) in resting and activated T_reg cells. Bars represent mean ± s.e.m. Data were analyzed using a Mann–Whitney U-test. b, Bar graphs showing the proportion of ATAC-seq peaks near Foxp3-dependent DEGs bound by Foxp3. Genes are stratified by P values in resting and activated T_reg cells. Bars represent mean ± s.e.m. Data were analyzed using a Mann–Whitney U-test. c, H3K27Ac and H3K27me3 ChIP-seq signals¹⁹ at Foxp3-bound ATAC-seq peaks near Foxp3 degradation-induced DEGs. Line graph represents mean ± s.e.m. Data were analyzed using a Mann–Whitney U-test. d, Dot plot showing transcription factor motif enrichment within Foxp3-bound regions near Foxp3 degradation-induced DEGs. e, Schematic diagram illustrating the ‘on’ and ‘off’ states of the reversible reporter-null Foxp3^LSL allele. f, Experimental design of the gain-of-function experiment to induce Foxp3 expression in T_reg ‘wannabe’ cells. Each genotype and time point consisted of two independent biological replicates. g, Line graph depicting gene expression changes of TIR1-up or TIR1-down genes below a specific P value cutoff across different time points following Foxp3 induction in T_reg ‘wannabe’ cells. The line and shading represent the mean ± s.e.m. Data were analyzed using a Mann–Whitney U-test.