Extended Data Fig. 6: Use of normalized intensity as opposed to extrapolated copy number. | Nature Immunology

Extended Data Fig. 6: Use of normalized intensity as opposed to extrapolated copy number.

From: Hypoxia induces histone clipping and H3K4me3 loss in neutrophil progenitors resulting in long-term impairment of neutrophil immunity

Extended Data Fig. 6: Use of normalized intensity as opposed to extrapolated copy number.

a, Total protein content obtained after applying the proteomic ruler method to calculate protein copy number and total intensity from liquid chromatography-mass spectrometry proteomic data from blood neutrophils in healthy donors compared to acute respiratory distress syndrome (ARDS) survivors 3–6 months post-hospital admission (n = 8 for both experimental groups). Data as mean ± s.d., with each value representing an individual. Significant p values depicted (for p < 0.05) and obtained by Shapiro-Wilk normality test followed by two-tailed t-test.

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