Extended Data Fig. 5: FS-scRNA-seq validation steps and creation of benchmark dataset.
From: A unified multimodal single-cell framework reveals a discrete state model of hematopoiesis in mice
a-f, Gating schemes derived from Ab-MarkerFinder with targeted enrichment for the MPP3-IER (a), ML-1b and ML-2 (b), MEP (c), BMCP (d), IG2-MP (e), and IG2-proNeu1 (f) clusters. g, InfinityFlow in silico gated populations replicating mutually-exclusive (non-overlapping) FS-scRNA-seq definitions projected over the UMAP embedding. h, Gene expression profiles of CITE-seq cells (cluster colors are the same as those pointed to in g) that map to FS-scRNA-seq sorted populations by their marker genes. i, Example heatmap of pairwise overlap between the true gate label to the predicted gate label (confusion matrix) in InfinityFlow data used to calculate ARI scores.
