Extended Data Fig. 9: MultiLin populations display distinct lineage priming.
From: A unified multimodal single-cell framework reveals a discrete state model of hematopoiesis in mice
a, sc-Hrödinger scores using the top 100 marker genes per cluster for mixed-lineage priming of the indicated cluster gene expression programs (top) across the indicated CITE-seq atlas populations (left) using CITE-seq gene expression data. b, Bar plots show the expression of specified progenitor marker genes within MultiLin clusters. c, Capybara predictions, using scaled quadratic programming (QP) and multiple identity (Multi-ID) percentages for each cell state relative to the same restricted cell-states as in (a). d, The integrated scRNA-seq gene expression UMAP illustrating PAGA-defined linkages (edges) between states colored as high confidence (blue), medium confidence (green), and not recapitulated by CellTag (dotted). e, CellTag workflow: Two progenitor subsets (Lin−Kit+Sca+, Lin−Kit+Sca−) were independently transduced with CellTag-multi lentiviral barcoding vector, cultured and then GFP+ cells were sorted and captured for scRNA-seq analysis (n = 2 technical replicates). f, Cells in MultiLin Lin−Kit+Sca−CD27+ gate were index-sorted for CD55−CD371−, CD55+CD371−, CD55−CD371+ populations and single cells were cultured for 5 days. The output of single-cells is classified as either (gray) all tdTomato− (red) all tdTomato+, or (purple) a mix of tdTomato+ and tdTomato− cells.
