Extended Data Fig. 10: Flow cytometric and genomic dissection of MultLin heterogeneity and lineage restriction.
From: A unified multimodal single-cell framework reveals a discrete state model of hematopoiesis in mice
a, 11-color Flow cytometry panel used with Sony MA900 sorter to monitor activity of lineage-defined-CRE activation of ROSA-LSL-tdTomato reporters. b, Heatmap comparison of the predicted cluster content of in-silico-sorted InfinityFlow cell populations (left) and FS-scRNA-Seq analysis (right) for the sort gates (A-G). c, Nippostrongylus brasiliensis infection model schematic shows full spectrum flow analysis and scRNA-seq capture timepoints after infection. d, Cell frequency curve plot for 4 out of 22 in vivo perturbation scRNA-seq datasets. MultiLin cell populations are re-scaled among themselves to 1 (left side of the plot). Unadjusted cell frequency is shown for selected non-MultiLin clusters. e, Chi-square residuals for MultiLin scaled cell-population frequency versus all non-HSPC-MultiLin clusters with significant associations (*).
