Extended Data Fig. 6: SATB1 downregulation is required for proper effector differentiation in acute viral infection.

(a-c) Naïve CD45.1⁺ P14 cells were adoptively transferred into naïve wildtype CD45.2⁺ mice and infected with LCMV-Armstrong. Spleens were harvested on days 7 or 28 p.i. and analyzed using flow cytometry. (a) Flow cytometry plots and quantification of SATB1-KO and control CX3CR1 and KLRG1 SLEC populations on day 7 p.i. (n = 10). (b) Histogram and quantification of GzmB producing SATB1-KO or control SLEC on day 7 p.i. (n = 10). (c) Quantification of SATB1-KO (n = 9) and control (n = 10) T_CM and T_EM cell numbers per spleen on day 28 p.i. (d-g) Mixed bone marrow chimeric mice containing Satb1^Tg (CD45.2⁺) and control T cells (CD45.1/2⁺) were infected with LCMV-Armstrong. On day 7 p.i. spleens were analyzed using flow cytometry. (d) Flow cytometry plots and quantification of activated CD44⁺ Satb1^Tg and control cells (n = 10). (e) Quantification of PD-1 expression in PD-1⁺ and Gp33⁺ Satb1^Tg and control cells (n = 10). (f) Flow cytometry plots and quantification of CX3CR1 and KLRG1 Gp33⁺ Satb1^Tg and control populations (n = 10). (g) Quantification of cytokine expression Satb1^Tg and control cells after Gp33 peptide restimulation (n = 10). Flow cytometry plots are representative. Dots in graphs represent individual mice; horizontal lines and error bars of bar graphs indicate means ± SEM, respectively. Data are pooled from two independent experiments. P values are from two-tailed paired student’s t test.