Extended Data Fig. 9: SATB1 limits tumor control and response to checkpoint inhibition. | Nature Immunology

Extended Data Fig. 9: SATB1 limits tumor control and response to checkpoint inhibition.

From: Transcriptional regulator SATB1 limits CD8+ T cell population expansion and effector differentiation in chronic infection and cancer

Extended Data Fig. 9: SATB1 limits tumor control and response to checkpoint inhibition.The alternative text for this image may have been generated using AI.

Naïve CD45.2+ SATB1-deficient P14 cells (Satb1flex/flex/Cd8Cre, SATB1-KO) or control cells (Satb1flex/flex) P14 cells were adoptively transferred into naïve wildtype CD45.1+ mice 3 days after inoculation with B16/Gp33+ Tumor cells. Mice were treated with immune checkpoint blockade (ICB, anti-PDL-1 + anti-CTLA4) or PBS. (a,b) Tumor growth (a) and survival (b) of mice transferred with either SATB1-KO or control P14 cells treated with or without ICB (n = 8). Data are pooled from two independent experiments. (c,d) Representative flow cytometry plots and quantification showing the frequencies of IFN-γ and TNF producing SATB1-KO and Control P14 cells in the tumor (c) and tdLNs (d) treated with or without ICB after ex vivo restimulation with Gp33 peptide (untreated n = 8, treated KO n = 5, treated WT n = 7). Dots in graphs represent individual mice; horizontal lines and error bars of bar graphs indicate means ± SEM, respectively. Data are pooled from two independent experiments. P values are from two-way ANOVA (b) or unpaired student’s t test (c,d).

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