Extended Data Fig. 10: SATB1 limits effector differentiation of CD8⁺ T cells in tumor draining lymph nodes.

Naïve CD45.2⁺ SATB1-deficient P14 cells (Satb1^flex/flex/Cd8^Cre, SATB1-KO) or control cells (Satb1^flex/flex) P14 cells were adoptively transferred into naïve wildtype CD45.1⁺ mice 3 days after inoculation with B16/Gp33 tumor cells. Mice were treated with immune checkpoint blockade (ICB, anti-PDL-1 + anti-CTLA4) or PBS on indicated days. Tumor draining LN (tdLNs) were harvested on day 16 after inoculation and analyzed using flow cytometry. (a) Representative flow cytometry plots and quantification showing frequencies and numbers of SATB1-KO or control P14 cells (untreated n = 8, treated KO n = 6, treated WT n = 7). (b) Representative flow cytometry plots and quantification showing frequencies and numbers SATB1-KO or control CX3CR1⁺ T_EX cells (untreated n = 8, treated KO n = 6, treated WT n = 7). (c) Representative flow cytometry plots and quantification showing frequencies and numbers of GzmB producing SATB1-KO or control P14 cells (untreated n = 8, treated KO n = 6, treated WT n = 7). Dots in graphs represent individual mice; horizontal lines and error bars of bar graphs indicate means ± SEM, respectively. Data are representative of two independent experiments. P values are from unpaired student’s t test.