Extended Data Fig. 1: SATB1 limits T_EX cell differentiation.

(a) Histogram and quantification showing the expression of SATB1 in polyclonal PD-1⁺ T_PEX and T_EX cells compared to naïve CD8 T cells and B cells (n = 10). (b-e) Naïve SATB1-deficient mice (Satb1^flex/flex/Cd8^Cre, SATB1-KO) or control mice (Satb1^flex/flex) were analyzed using flow cytometry. (b) Flow cytometry plots displaying GFP expression upon Cre-mediated Satb1 gene flip in CD8⁺ T cells of KO mice compared to control mice (n = 5). (c) Quantification of SATB1 expression in CD4⁺ and CD8⁺ T cells (n = 5). (d) Numbers of splenic CD4⁺ and CD8⁺ T cells in SATB1-KO and control mice (n = 5). (e) Flow cytometry plots and quantification showing CD44 vs CD62L expression in SATB1-KO and control mice and quantification of naïve and memory phenotype CD8⁺ T cells (n = 5). (f-i) Naïve CD45.2⁺ SATB1-KO P14 cells or control cells P14 cells were adoptively transferred into naïve wildtype CD45.1⁺ mice and subsequently infected with LCMV-Docile. On day 12, 21, and 28 p.i. spleens were analyzed using flow cytometry. (f) Flow cytometry plots showing frequencies and numbers of SATB1-KO or control T_PEX and T_EX cells. (g) Flow cytometry plots and quantification on days 12 (upper) (n = 8) and 21 (lower) (n = 14) p.i. showing frequencies and numbers of SATB1-KO or control CD62L⁺ T_PEX cells. (h) Flow cytometry plots and quantification on day 28 p.i showing the frequencies and numbers of SATB1-KO or control P14 cells (n = 5). (i) Flow cytometry plots and quantification on day 28 p.i showing the numbers of SATB1-KO and control T_PEX, total T_EX, CX3CR1⁺ T_EX and CD101⁺ T_EX cells (n = 5). Flow cytometry plots are representative. Dots in graphs represent individual mice; horizontal lines and error bars of bar graphs indicate means ± SEM, respectively. Data are pooled (a,f,g) or representative (b-e, h,i) from two (day 12, day 28 p.i.) or three (day 21 p.i.) independent experiments. P values are from two-tailed unpaired student’s t test.