Extended Data Fig. 5: TCM-derived PS activates TIM4-AMPKα signaling to suppress TRAP1 expression.
From: Cancer suppresses mitochondrial chaperone activity in macrophages to drive immune evasion
(a) Phosphatidylserine (PS) levels in YUMM1.7 TCM were measured by ELISA (n = 4). Data are representative of three independent experiments. (b-e) BMDMs were cultured with either YUMM1.7 TCM or apoptotic YUMM1.7 cells (AC) for 24 h in the presence of IgG2b or αTIM4, and the expression levels of TIM4 (b), p-AMPKα (c), TRAP1 (d), and ARG1 (e) were analyzed by flow cytometry (n = 4). Data are representative of three independent experiments. (f-i) BMDMs were co-cultured with either PS or TCM for 24 h, and expression levels of TIM4 (f), p-AMPKα (g), TRAP1 (h) and ARG1 (i) were determined by flow cytometry (n = 4). Data are representative of three independent experiments. All data are mean ± s.e.m. and were analyzed using a two-tailed, unpaired Student’s t-test (a) or a one-way ANOVA with Sidak’s multiple comparison test (b-i).
