Fig. 1: CD11b+ myeloid cells migrate from the skin to joints in a model of psoriatic disease. | Nature Immunology

Fig. 1: CD11b+ myeloid cells migrate from the skin to joints in a model of psoriatic disease.

From: Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints

Fig. 1: CD11b+ myeloid cells migrate from the skin to joints in a model of psoriatic disease.The alternative text for this image may have been generated using AI.

a, Left, representative micrographs of H&E-stained skin sections of the hind paws of BALB/c and C57BL/6 mice at day 21 with and without IL-23OE. Right, quantification of epidermal thickness at day 21. The graph shows the median, quartiles and minimum–maximum; N = 4 per condition. P values were calculated by one-way analysis of variance (ANOVA) with a Tukey’s post hoc test. b, Left, representative micrographs of H&E-stained ankle sections of BALB/c and C57BL/6 mice at day 21 with and without IL-23OE. Right, quantification of arthritis at day 21. The graph shows the median, quartiles and minimum–maximum; N = 8 per condition. P values were calculated by one-way ANOVA with a Tukey’s post hoc test. c, Representative micrographs of MRI-scanned ankles of BALB/c and C57BL/6 mice at day 21 with and without IL-23OE used for the quantification of arthritis at day 21. Arrowheads indicate inflammation, and stars indicate the talar bone. The graph shows the median, quartiles and minimum–maximum; N = 5 per condition. P values were calculated by one-way ANOVA with a Tukey’s post hoc test; T1w CE, T1-weighted contrast-enhanced. d, Representative micrographs of light sheet fluorescence microscopy of Kaedetg ankles from BALB/c (C-Kaedetg) and C57BL/6 (B6-Kaedetg) background strains at day 7 with and without IL-23OE and after photoconversion of cells localized in the skin. Arrowheads indicate accumulations of photoconverted KaedeRED cells. Graphical drawings of the tibia (Ti), calcaneus (Ca) and Achilles tendon (Te) are included. e, Representative flow cytometry plots for the quantification of KaedeRED skin-derived cells in the joint. f, Quantification of KaedeRED skin-derived cells in the joints. The graph shows the mean and standard error of the mean; N = 4 per time point and condition. g, Representative micrographs of imaging flow cytometry for the typing of KaedeRED skin-derived cells in the joint at day 7; BF, brightfield. h, Quantification of CD45+ KaedeRED skin-derived cell types in the joints at day 7. The graph shows medians, quartiles and minimum–maximum; N = 4 per condition. P values were calculated by one-way ANOVA with a Tukey’s post hoc test.

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