Extended Data Fig. 1: Assessment of psoriasis and arthritis over time.
(a) Macroscopic evaluation of skin-scaling in mice with/without IL-23OE for 21 days (left). Total scaling quantified as area under the curve (AUC, right). Graphs: mean ± SEM (left) and median, quartiles, min-max (right); N = 12 per condition. P values calculated by one-way ANOVA with Tukey´s post hoc test. (b) Histological inflammation scores for ankle regions based on H&E staining in mice with/without IL-23OE for 21 days (left). Quantification of arthritis as AUC (right). Graphs: mean ± SEM (left) and median, quartiles, min-max (right); N = 8 per time point and condition. P values calculated by one-way ANOVA with Tukey´s post hoc test. (c) MRI ankle measurements over time in mice with/without IL-23OE for 21 days (left). Quantification of arthritis as AUC (right). Graphs: mean ± SEM (left) and median, quartiles, min-max (right); N = 5 per condition. P values calculated by one-way ANOVA with Tukey´s test. (d-f) Representative micrographs of H&E-stained sections and representative photographs from skin of ear and tail (d), distal paws (e) and ankles (f) of mice at day 21 with/without IL-23OE. In (e): dashed lines highlight dactylitis; S = skin, B = bone. In (f): dashed squares in the overview identify the areas of corresponding magnifications; black arrows indicate sites of inflammation. Images representative for three independent experiments with similar results. (g) Histological inflammation score of synovia in ankle and tarsal joints based on H&E-stainings in mice with/without IL-23OE for 21 days. Graphs: median, quartiles, min-max.; N = 4 per condition; P values calculated by one-way ANOVA with Tukey´s post hoc test. (h) Micro-CT quantification of tibia from mice at day 21 with IL-23 OE. BV/TV = bone volume/total volume; Tb. N = trabecular number; Tb. Th = trabecular thickness. Graphs: median, quartiles, min-max; N = 10 per condition; P values calculated by one-way ANOVA with Tukey´s post hoc test. (i) Representative micro-CT images of the tibial trabecular network of mice at day 21 with/without IL-23 OE. (j) Representative micro-CT images from hind paws of mice at day 21 with/without IL-23OE. Red and green dashed squares indicating corresponding magnification of metatarsal bone and calcaneus.
