Fig. 1: CAR costimulatory domains control functional effect of tonic signaling.
From: BACH2 regulates T cell lineage state to enhance CAR T cell function
a, Schematic of the four CAR constructs generated to test the impact of tonic signaling. b, CAR T cell expansion during manufacture (NTDs). c, PD1 expression during CAR T cell manufacture. d, Nalm6 cell survival over time in co-culture with CAR T cells. e, Quantification of cytokines in culture supernatants 48 h after combination of Nalm6 and CAR T cells. f, CAR T cell expansion at day 6 of co-culture with Nalm6. g, Expression of PD1, TIM3 and LAG3 by CAR T cells over the course of co-culture with Nalm6. For b–g, representative data are from n = 4 donors. All data are presented as either individual values or mean values ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001 by two-way analysis of variance (ANOVA) with Bonferroni’s adjustment for multiple comparisons. IFNγ, interferon γ; IL-2, interleukin-2; MFI, mean fluorescence intensity; NS, not significant; TNF, tumor necrosis factor. Schematic in a created with BioRender.com.
