Extended Data Fig. 4: Agonist-selected T cells in CD8^Dual mice.

a. Foxp3 (ic) and CD25 staining among mature CD8.2 and CD8 thymocytes from CD8^Dual and B6 mice, related to Fig. 2c. b. CD1d-TET and TCRβ staining of whole thymocytes from CD8^Dual and LM control WT mice, related to Fig. 2d. c. Flow cytometry analysis of CD1d-TET⁺CD24⁻ thymic mature NKT cells from CD8^Dual (green) and LM control WT (gray) mice (n = 3/strain, representative of 3 independent experiments). d. Intracellular staining (ic) of T-bet in NKT subsets from CD8^Dual and LM control WT mice (n = 3/strain, representative of 3 independent experiments). e. Intracellular staining (ic) of Gata3 in NKT subsets from CD8^Dual and LM control WT mice (n = 3/strain, representative of 3 independent experiments). f. IFN-γ, IL-4, and IL-17 expression in CD1d-TET⁺CD24⁻ thymic mature NKT cells from CD8^Dual and LM control WT mice. Whole thymocytes were cultured with medium or PMA+Ionomycin in the presence of golgi stop for 4 h and assessed for cytokine production (n = 3/strain, representative of 3 independent experiments). Numbers within profiles indicate frequency of cells in each box (a-c,f).