Fig. 2: Activation of Notch signaling in macrophages promotes an antitumor effect.
From: Exclusion of Notch from the contact site during efferocytosis restricts anticancer immunity
a, Tumor growth of Yumm1.7 cells in LysM-cre−;loxp-STOP-loxp (LSL)-NICD (WT, n = 9) and LysM-cre+;LSL-NICD (NICD overexpression (OE), n = 5) mice. All WT mice were littermates of NICD OE mice. Data are the mean ± s.e.m. Statistical analysis was conducted using a two-way ANOVA. b, Tumors from mice analyzed in a were collected and weighed (n = 6 for cre− mice; n = 4 for cre+ mice). c, Absolute cell number of CD4+ T cells and CD8+ T cells in each tumor in b. d, Representative flow cytometry plots and frequency of IFNγ+TNF+ among tumor-infiltrated CD8+ T cells (n = 6 for cre− mice; n = 4 for cre+ mice). e, Volcano plot of preranked GSEA results using gene sets from MSigDB against log2(fold change) for NICD OE versus WT. The y axis is the −log10(P value) with a pseudovalue of 1 × 10−5 for 0. The x axis is the normalized enrichment score (NES). The horizontal dashed line represents P = 0.05. f, GSEA of hallmark IFNα response genes in TAMs. g, Heat map showing IFNα response genes that are upregulated in TAMs from NICD OE mice. Data are the mean ± s.d. Statistical analysis was conducted using a Student’s t-test (b–d). GSEA significance was calculated using one-sided permutation testing based on a preranked approach.
