Extended Data Fig. 5: Runx1-deletion results in enhanced surfactant gene expression.
From: Thymic alveolar type 2 epithelial mimetic cells revealed by RUNX1 deficiency
a-d, Volcano plots of DGE from scRNA-seq analysis of Foxn1-Cre(+)/Runx1+/+ (WT) mice and Foxn1-Cre(+)/Runx1fl/fl (KO) mice for Ccl21alow (a), Late Aire (b), Keratinocyte-like mTECs (c), and M cell mTECs (d). e, Confocal images of SFTPC (green), DCLK1 (red), and DAPI (blue) from the thymus of Foxn1-Cre (-)/Runx1fl/fl (Cre(-)) and Foxn1-Cre(+)/Runx1fl/fl (Cre(+)) mice. Images taken at 63X magnification. Scale bars = 25 μm. f, Confocal images of SFTPC (green), KRT10 (red), and DAPI (blue) from the thymus of Cre(-) and Cre(+) mice. Images taken at 190X magnification. Scale bars = 15 μm. g-i, Violin plots of chemokine gene expression from scRNA-seq. (a-d) Differentially expressed genes in volcano plots were determined using a two-sided Wilcoxon rank-sum test. (e,f) Data are representative of three independent experiments.
