Extended Data Fig. 6: Runx1-deletion in TECs does not alter SP or DP thymocyte populations.
From: Thymic alveolar type 2 epithelial mimetic cells revealed by RUNX1 deficiency
a, Flow cytometry and percentage/absolute number of thymic single positive (SP) CD4+ T cells, SP CD8+ T cells, double positive (DP) T cells, and double-negative (DN) T cells from 30-day-old Foxn1-Cre(-)/Runx1fl/fl mice (Cre(-)) and Foxn1-Cre(+)/Runx1fl/fl mice (Cre(+)) (n = 8 mice; two independent experiments). b, Flow cytometry of B220−/Nk1.1−/TCRγδ−/CD25−/CD5+/TCRβ+ signaled and B220−/Nk1.1−/TCRγδ−/CD25−/CD5−/TCRβ− non-signaled T cells Cre(-), Cre(+), and Foxn1-Cre(+)/Ikzf1fl/fl (Ikzf1fl/fl) mice (n = 3 Cre(-), n = 5 Cre(+), and n = 2 Ikzf1fl/fl mice; two independent experiments). c, H&E staining of the lacrimal gland, and salivary gland. Scale bars = 500 μm. Bar graphs quantitate lymphocytic infiltrate (n = 5 Cre(-) and n = 4 Cre(+) mice). d, Merged UMAP of scRNA-seq data from Foxn1-Cre(+)/Runx1fl/fl (Cre(+)) mTECs, Aire WT mTECs, and Aire−/− mTECs. Approximately 12,000 mTECs were sequenced for both the Aire WT and Aire−/− datasets. e, Overlay of cells from each genotype on the merged UMAP. Red numbers represent the percentage of AT2 mTECs in each genotype. f, Bar graphs representing the percentage of mimetic cells found in Aire WT vs Aire−/− mTECs. (a-c) In graphs, the bars correspond to the mean, with error bars showing ±SD of values shown, and each data point represents an individual mouse. (a-b) P values by unpaired two-tailed t-test. (c) P value by two-tailed nonparametric Mann Whitney.
