Extended Data Fig. 2: Runx1-deletion in TECs does not alter thymic tuft cells.
From: Thymic alveolar type 2 epithelial mimetic cells revealed by RUNX1 deficiency
a, Confocal microscopy of cytokeratin 5 (KRT5) (red), Aire (green), and DAPI (blue) of 30-day old thymi from Foxn1-Cre (-)/Runx1fl/fl (Cre(-)) and Foxn1-Cre(+)/Runx1fl/fl (Cre(+)) mice. Images taken at 20X magnification and scale bar = 25 μm. Inserts taken at 200X magnification and scale bar 5 μm. b-c, Flow cytometry (left) and percentage and absolute number (right) of Ki67+ mTECs gated on CD11c−/CD45−/EpCAM+/Ly51−/IAb+ TECs (b) or Ki67+/Aire+ mTECs gated on CD11c−/CD45−/EpCAM+/Ly51−/IAb+/Aire+ mTECs (c) (n = 6 mice; two independent experiments). d, Flow cytometry (left) and percentage and absolute number (right) of Apotracker+ mTECs gated on CD11c−/CD45−/EpCAM+/Ly51−/IAb+ mTECs (n = 4 mice; two independent experiments). e-f, Flow cytometry (left) and percentage and absolute number (right) of DCLK1+ thymic tufts cells (e), L1CAM+ thymic tufts cells (f) gated on CD11c−/CD45−/EpCAM+/Ly51−/IAb+ TECs (n = 3 (e) and n = 4 (f) mice; (e) representative experiment, and (f) two independent experiments). g, Confocal microscopy of cytokeratin 10 (KRT10) (red), DCLK1 (green), and DAPI (blue) of 30-day old thymi. Images taken at 20X magnification. Scale bars = 25 μm. (b-f) In graphs, the bars correspond to the mean, with error bars showing ±SD of values shown, and each data point represents an individual mouse. (b-f) P values by unpaired two-tailed t-test. (a,g) Data are representative of three independent experiments.
