Supplementary Figure 4: Comparison of label fragmentation versus peptide backbone fragmentation.
From: EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
(a) EASI- and TMT-labeled HeLa peptides were fragmented with normalized collision energies between 10 and 34. ΔNCE was calculated as the difference of label fragmentation versus peptide backbone fragmentation (N = 10,321 precursors for EASI and 12,875 for TMT). Boxplot elements: center line = median, lower and upper box limits = first and third quartiles, whiskers = maximum 1.5x inter-quartile range.
