Supplementary Figure 6: Distribution of ratios and coefficients of variation for EASI-tagged peptides.
From: EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
(a) Distribution of peptide-coupled reporter ion ratios (log10) in MS/MS scans of yeast (mixing ratio 1:3:10:10:3:1) and human (mixing ratio 1:1:1:1:1:1) peptides (N=369,591, 375,384, 377,496, 377,839 and 378,101 for human channels 0 to 4 and 224,098, 209,244, 146,253, 152,255 and 215,707 for yeast channels 0 to 4). (b) Distribution of the coefficients of variation for EASI-tag quantified yeast and human peptides in three replicate injections (N= 39,400, 39,827, 39,898, 39,930 and 39,882 unique sequences for human channels 0 to 4 and 20,738, 19,947, 15,657, 16,097 and 20,230 unique sequences for yeast channels 0 to 4).
