Supplementary Figure 4: Orthogonal StaPL modules allow for independent, simultaneous control of nuclear localization for two fluorescent proteins.
From: StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
Top, schematics of constructs used for small molecule control of nuclear localization. Tandem YFPs or RFPs were fused to a nuclear localization signal (NLS), with an intervening StaPL module and cleavage site governing preservation of the NLS. Constructs were transiently expressed in HEK293A cells and incubated 8-12 h in the indicated drugs. Left, for tdYFP-StaPLAI-NLS, concentrated nuclear YFP fluorescence is observed for ASV but not for TPV or DMSO vehicle. For tdRFP-StaPLTI-NLS, concentrated nuclear RFP fluorescence is observed in TPV but not for ASV or vehicle. Right, the opposite sensitivity to TPV and ASV is observed with tdYFP-StaPLTI-NLS and tdRFP-StaPLAI-NLS. Representative confocal images of live cells acquired 8-12 h after transfection are shown. Scale bars, 15 μm. Data represent a single experiment.
