Supplementary Figure 7: Effect of attenuated MEK inhibition on X chromosome inactivation status.
From: Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells
(a) Representative RNA FISH images for primed and naïve UCLA5 at P16, detecting XIST and nascent transcription foci of UTX and HUWE1 (scale bar: 10 μm). (b) Representative RNA FISH images for primed and naïve UCLA1 at P16, detecting XIST and nascent transcription foci of UTX and HUWE1 (scale bar: 10 μm). Note that UCLA1 hESCs are often monoallelic for UTX. (c) Table summarizing the HUWE1 status in the different primed and naïve hESC lines analyzed.
