Supplementary Figure 3: Alternative MEK inhibitors maintain naive pluripotency in hESCs cultured in t2iLGöY. | Nature Methods

Supplementary Figure 3: Alternative MEK inhibitors maintain naive pluripotency in hESCs cultured in t2iLGöY.

From: Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells

Supplementary Figure 3: Alternative MEK inhibitors maintain naive pluripotency in hESCs cultured in t2iLGöY.

(a) Clonal colony formation assay of hESCs cultured in t2iLGöY supplemented with the indicated concentration of MEKi. Shown are representative images of alkaline phosphatase-positive hESC colonies. (b) Quantification of alkaline phosphatase-positive hESC colonies shown in Supplementary Figure 2A. (c) Quantitative RT-PCR analysis of OCT4 and NANOG expression using indicated samples. Error bars indicate mean ± s.d., n = 3 biologically independent samples (cell cultures). (d) Daily quantification of cell numbers during the expansion of naive hESCs in t2iLGöY for 5 passages.

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