Supplementary Figure 10: Mild fixation conditions favor SOMAmer binding to GFP.
From: Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
Four different paraformaldehyde (PFA) concentrations and incubation times were evaluated for GFP SOMAmer staining intensity on HeLa Kyoto 2xZFN mEGFP–Nup107 cells. We saw increased SOMAmer-Cy3 signal (top row) for short incubation time and low PFA concentration. Unchanged signal from GFP (bottom row) shows preservation of the Nup107 protein for all tested fixation conditions. Scale bar: 15 µm.
