Supplementary Figure 11: Addition of low-molecular-weight dextran sulfate blocks unspecific nuclear SOMAmer binding.
From: Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
Fixed A549 cells were incubated with 100 nM of EGFR SOMAmer in SOMAmer staining buffer containing 100 µM Z-Block and either 1 µM (left) or 100 µM (right) dextran sulfate. The increase of dextran sulfate concentration led to a drastic decrease of signal in the nucleus. Scale bar: 20 µm.
