Supplementary Figure 12: Addition of Z-Block quenches unspecific binding of SOMAmers to cellular organelles.
From: Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
Fixed HeLa Kyoto 2xZFN mEGFP–Nup107 cells were incubated with 100 nM of EGFR SOMAmer in SOMAmer staining buffer containing 1 mM dextran sulfate and either 10 µM (top) or 100 µM (bottom) Z-Block. The increase of Z-Block concentration led to a drastic decrease of signal from cellular organelles. Scale bar: 10 µm.
