Supplementary Figure 8: Tricolor labeling of chromosome 19 loci by CRISPR-Sirius.
From: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
(a) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7 and sgRNA-Sirius-4X(MS2-PP7). (b) Top shows the diagram of chromosome-19-specific repeats located at the subtelomeric region of p-arm (T1) and q-arm (T2), and also the pericentromeric region (PR1), which were targeted by CRISPR sgRNA-Sirius-4XMS2-4XPP7, Sirius-8XMS2 and Sirius-7XPP7, respectively. Bottom shows simultaneous labeling the loci T1 (yellow), PR1 (green) and T2 (red) by the merged fluorescence (right panel). Phase-contrast images and fluorescent images overlaid are shown (left panel). Data in all panels are representative of experiments performed at least three times.
