Supplementary Figure 12: CRISPR-Sirius labeling of chromosome-19-specific loci.
From: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
(a) Schematic of seven unique loci on human chromosome 19 used in this study consisting of 4 intergenic DNA regions (IDRs), 2 intronic regions (TCF3 and FBN3) and 1 pericentromeric region (PR1). (b) Diagrams of CRISPR sgRNA-Sirius-8XMS2 and sgRNA-Sirius-8XPP7 (upper panels) and live cell imaging of locus pairs between IDR1/IDR3, IDR2/IDR3, TCF3/IDR3, IDR4/IDR3, FBN3/IDR3 and PR1/IDR3 (low panels). Data in all panels are representative of experiments performed at least three times. (c) Time lapse of IDR3/IDR3 tracked in a single cell over 2 min. Data in all panels are representative of experiments performed at least three times.
