Supplementary Figure 3: Optimization of the structure and labeling efficiency of MS2 octets for CRISPR imaging. | Nature Methods

Supplementary Figure 3: Optimization of the structure and labeling efficiency of MS2 octets for CRISPR imaging.

From: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging

Supplementary Figure 3: Optimization of the structure and labeling efficiency of MS2 octets for CRISPR imaging.

(a–c) Diagrams of sgRNAs-In-8XMS2, sgRNAs-In-8XMS2T and sgRNA-Sirius-8XMS2 bound with MCP-HaloTag. Different colors of MS2 shown in Sirius-8XMS2 (c) represent distinct modifications in each hairpin. (d) Typical images of labeling intronic repeats (22 copies) of the FBN3 gene (upper panel) using sgRNA-In-8XMS2 (left panel), sgRNA-In-8XMS2T (middle panel) and sgRNA-Sirius-8XMS2 (right panel). Data in all panels are representative of experiments performed at least three times. (e) The number of foci per cell for FBN3 loci using the sgRNAs-In-8XMS2, In-8XMS2T and Sirius-8XMS2. The line within the box plot represents the mean; the outer edges of the box are the 10th and 90th percentiles; the whiskers extend to the minimum and maximum values; n = 73, 83 and 88 cells from left to right.

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