Supplementary Figure 5: Comparison of labeling efficiency of sgRNA-3′-14XMS2 and sgRNA-Sirius-8XMS2.
From: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
(a) Diagram of sgRNA-3′-14XMS2 and sgRNA-Sirius-8XMS2 with the dCas9-GFP and BFP reporter. (b) Visualization of C19-1 (a ~1 kilobase subtelomeric region on the short arm of chromosome 19). Localization of BFP report (blue), dCas9-GFP (green), and C19-1 foci (red and indicated by the arrows) in U2OS cells. Scale bar, 5 μm. The images with the same color were scaled to the same minimal and maximal levels. Data in all panels are representative of experiments performed at least three times. (c) The number of foci per cell for C9-1 loci using the sgRNAs-3′-14XMS2 and Sirius-8XMS2. The line within the box plot represents the mean; the outer edges of the box are the 10th and 90th percentiles; the whiskers extend to the minimum and maximum values; n = 94 cells for sgRNAs-3′-14XMS2 and 125 cells for sgRNA-Sirius-8XMS2.
