Supplementary Figure 5: Light exposure did not silence tonic firing activity of neurons from mouse DRN injected with a GFP control virus.
From: A light-gated potassium channel for sustained neuronal inhibition
(a) (Left) Diagram of injection site in the DRN. (Right) Sample confocal image showing expression of the AAV1/2-hSyn-eGFP virus in the DRN (green, GFP; gray, DAPI) (image representative of n = 3). Scale bars, 200 µm or 40 µm (inset). AQ, aqueduct. (b) (Top, left) Representative cell-attached voltage clamp recording of firing response before and after 60-s blue light illumination (blue bar) (n = 7 independent recordings). (Top right) Expanded view of the recording period defined by the red dotted box. (Bottom left) Time course of the effect of 60-s blue light stimulation (blue bar) on discharge firing rate (5-s binning). (Bottom right) Summary plots indicate mean firing discharge rate 2 min prior to light (Beforelight) and 2 min after lights-off (Afterlight 0–2′) (Beforelight, 3.4 ± 0.8 Hz; Afterlight 0–2′, 4.1 ± 1.0 Hz n = 7, P = 0.46, t = 0.78, df = 6, two-sided paired t-test). Data are presented as mean ± s.e.m.
