Supplementary Figure 6: Passive and active membrane properties in mouse DRN BLINK2-expressing and GFP-expressing neurons.
From: A light-gated potassium channel for sustained neuronal inhibition
(a) (Left) Representative current traces. (Right) Membrane resistance (Rm) was measured in response to –5-mV steps in voltage clamp configuration, and did not significantly change between BLINK2 and CTRL group (Rm, BLINK2 386 ± 35 MΩ, n = 14; CTRL, 308 ± 35 MΩ, n = 15; P = 0.12, t = 1.59, df = 27, two-sided unpaired t-test). (b) (Left) Representative current clamp traces in response to fixed current injections. Action potential firing was generated by 2-ms current pulses at 1.2 nA. (Right) resting membrane potential (R.M.P.) and the action potential threshold did not differ between BLINK2 and CTRL groups (R.M.P.: BLINK2, –50.6 ± 1.8 mV n = 7; CTRL, –50.9 ± 1,8 mV, n = 15, P = 0.90, t = 0.13, df = 20, two-sided unpaired t-test; Threshold: BLINK2, –31.1 ± 2.3 mV, n = 8; CTRL, –35.4 ± 1.9 mV, n = 15, P = 0.18, t = 1.39, df = 21, two-sided unpaired t-test). Action potential threshold was determined from the second derivative of the spike waveform. Data are presented as mean ± s.e.m. obtained from: BLINK2, independent recordings, n = 14, n = 6 mice; CTRL, independent recordings, n = 15, n = 4 mice.
